BacSeq – Bacterial sequencing at its most powerful

Accurate identification of bacterial isolates is critical to environmental monitoring programs. Genotypic methods, such as comparative sequencing of the 16S ribosomal RNA (rRNA) gene in bacteria, have been proven to be the most accurate and reproducible methods for identifying unknown organisms. The technology is inherently stable, which allows for reproducible data for identification.

For many organisms, traditional phenotypic identification is problematic, takes a great amount of time and can result in subjective interpretation of the test results.Not all strains within a given species consistently exhibit a particular characteristic, thereby limiting phenotypic identification methods. Additionally, library databases utilized in support of phenotypic identification are limited.

The Accugenix genotypic analysis process adheres to the reference method used by taxonomists because it delivers the most accurate and reliable sequence data for identifications. When this process is combined with a full coverage, validated library, the resulting phylogenic analysis is unparalleled in the industry.

The AccuGENX-ID® BacSeq identification process is FAST and ACCURATE!

Start with a Simple Source: A Single Isolated Colony, Ethanol Extract, DNA Extract or FTA Micro Elute Card
Extract the genomic DNA from the isolated colony
Use PCR to amplify the first 500 base pairs of the 16S gene
Perform cycle sequencing to fluorescently label the DNA
Visualize the sequence data on an automated sequencer
Analyze the data against a proprietary database
Generate an ID Report
Quality Assurance review of Report
Technical Support issue of report to the customer 98% of Samples Reported Out 0.2% Error Rate